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HIV:Diagnosis Of HIV -1/2 Infection In Individuals > 18 Months Old

HIV-1/2 is diagnosed either by detecting HIV- specific antibodies or the virus itself. In individuals >18 months old the detection of HIV-specific antibodies remains the recommended method for routine diagnosis.

A) Antibody Detection

  • 4th generation HIV-1/2 ELISA Detects HIV-specific antibodies and p24 antigen (a component of the virus) simultaneously. Window period: ± 18 days.
  • Rapid HIV-1/2 Detects HIV-specific antibodies. (There are many different rapid tests available. Only those with
  • high sensitivity and specificity, and FDA/CE marking should be used.) Window period: ± 24-28 days.
  • According to WHO guidelines, all positive HIV results should be confirmed with: A second test on the same specimen.
  • Repeat testing on a second specimen. (Although the ELISA has a sensitivity of close to 100% and specificity of ± 99.6%, the serious nature of HIV warrants confirmatory testing.)

B) P24 Antigen Detection

  • The p24 antigen is detectable from approximately 2 weeks after infection.
  • During the seroconversion phase it is detected in the majority of patients.
  • However, when high levels of HIV-specific antibodies develop, the p24 antigen usually becomes undetectable.
  • A negative p24 result does not exclude HIV infection. Specific p24 antigen testing may be used to detect an acute infection, but should not be used for routine diagnosis. 

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